Tag

Part:BBa_K3581003:Design

Designed by: HUI Wan Hei   Group: iGEM20_HKUST   (2020-10-13)


HlyA signal peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

HindIII cut sites are flanking the coding region, allowing the signal peptide to be cut out so secretion method is changed from extracellular to inclusion bodies.


Source

Original sequence by iGEM09 Utah State.

References

1. Palmer, I., & Wingfield, P. T. (2004). Preparation and extraction of insoluble (inclusion-body) proteins from Escherichia coli. Current protocols in protein science, Chapter 6, Unit–6.3. https://doi.org/10.1002/0471140864.ps0603s38